RNA体内转染EntransterTM-in vivo – 英格恩中国官网

货号	规格	单价	数量
18668-11-1	0.5ml	¥2,599.00	RNA体内转染EntransterTM-in vivo 数量	加入购物车

产品介绍

Entranster^TM-in vivo是英格恩生物公司（Engreen Biosystem Co,Ltd.）最新研发合成的用于动物体内转染的转染试剂。Entranster^TM-in vivo动物体内转染试剂可将小片断RNA如siRNA，miRNA，mimic、inhibitor转染到动物体内，在动物体内进行RNA干扰等实验。

Entranster^TM-in vivo试剂不是病毒，改变了病毒转染方法复杂、周期长、不稳定、花费大、安全性等诸多问题。Entranster^TM-in vivo转染效率高，方法简单，无需病毒包装，直接将RNA送入目的细胞的细胞核中表达。

Entranster^TM-in vivo，非脂质体，采用纳米技术合成，通过保护和浓缩核酸，避免核酸被体液破坏，并通过物理作用将核酸富集在细胞表面，增强核酸进入细胞组织，从而产生作用。Entranster^TM-in vivo能耐受体内复杂的环境，不产生免疫反应。

主要特点

最快3天可以得到结果，核酸+转染试剂，注射动物，完成转染。

● 肝、肾、心、肺，血液系统，神经系统，生殖系统，皮肤…成功应用。

● 可方便、快捷更换不同的小片断RNA进行动物试验。

● 方法先进，抛弃病毒，众多实验室应用，稳定可靠。

● 比使用病毒节省90%以上费用，时间缩短到3天！

方法简单

如图所示，只需要将核酸和转染试剂分别稀释，混合，无需过滤，即可直接注射动物。

转染效果

将Entranster^TM-in vivo与Ambion in vivo siRNA(作用于凝血因子VII)和阴性siRNA通过尾静脉注射成年小鼠，2天后，取动物肝脏检测。在mRNA水平和蛋白水平观察干扰效果。见上图，图中最左侧组为对照组注射阴性siRNA为3mg/kg，后边3组为每kg动物注射阳性siRNA量分别为1mg/kg,2mg/kg和3mg/kg情况。

各器官转染

根据推荐用量注射Entranster^TM-in vivo和siRNA（作用于Lamin A/C）和阴性siRNA到成年小鼠。注射后2天收集相应的组织，分离RNA，用qRT-PCR分析Lamin A/C基因的表达水平。图4为尾静脉结果，图5为各器官分别局部注射结果。

应用本产品文献

发表的部分文献分器官导读

在线查看文章：https://files.engreen.info/share/detail/8fbe069f-6e6f-4937-7461-b2e1519bf000 提取码：btv9

序号	研究方向	实验材料	文章题目	期刊
大脑
1	大脑皮层	miRNA mimic and inhibitor	Significant changes in circular RNA in the mouse cerebral cortex around an injury site after traumatic brain injury	Experimental Neurology
2	大脑（阿尔兹海默症）	miRNA	MicroRNA-23b attenuates tau pathology and inhibits oxidative stress by targeting GnT-III in Alzheimer’s disease	Neuropharmacology
3	大脑海马	shRNA	Chemokine CXCL13 acts via CXCR5-ERK signaling in hippocampus to induce perioperative neurocognitive disorders in surgically treated mice	Journal of Neuroinflammation
4	大脑海马	siRNA	Silencing of PTEN inhibits the oxidative stress damage and hippocampal cell apoptosis induced by Sevoflurane through activating MEK1/ERK signaling pathway in infant rats	Cell Cycle
5	血脑屏障	siRNA	Semaphorin 3A Contributes to Secondary Blood–Brain Barrier Damage After Traumatic Brain Injury	Frontiers in Cellular Neuroscience
6	脑缺血损伤	agomiRNA	miR‑183‑5p attenuates cerebral ischemia injury by negatively regulating PTEN	Molecular Medicine REPORTS
7	脑缺血损伤	miRNA agomir	Inhibition of miR-200b-3p alleviates hypoxia-ischemic brain damage via targeting Slit2 in neonatal rats	Biochemical and Biophysical Research Communications
8	脑损伤	miRNA inhibitor	The role of microRNA-146a in regulating the expression of IRAK1 in cerebral ischemia-reperfusion injury	Canadian Journal of Physiology and Pharmacology
9	脑水肿	dsRNA	Effect of AQP4-RNAi in treating traumatic brain edema: Multi-modal MRI and histopathological changes of early stage edema in a rat model	EXPERIMENTAL AND THERAPEUTIC MEDICINE
10	大脑神经	siRNA	Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate is chemic neuronal death by targeting miR-21/PDCD4 signaling pathway	Cell Death and Disease
11	大脑神经	miRNA	miR-21 regulates ischemic neuronal injury via the p53/Bcl-2/Bax signaling pathway	AGING
神经
1	背根神经	shRNA	Dihydromyricetin Alleviates Diabetic Neuropathic Pain and Depression Comorbidity Symptoms by Inhibiting P2X7 Receptor	Frontiers in Psychiatry
2	周围神经	siRNA	Suppressing BRD4 exhibits protective effects against vincristineinduced peripheral neuropathy by alleviating inflammation and oxidative stress	Biochemical and Biophysical Research Communications
脊髓
1	自身免疫性脑脊髓炎	miRNA	miR‑20a suppresses Treg differentiation by targeting Map3k9 in experimental autoimmune encephalomyelitis	Journal of Translational Medicine
2	创伤性脊髓损伤	siRNA	MANF attenuates neuronal apoptosis and promotes behavioral recovery via Akt/MDM-2/p53 pathway after traumatic spinal cord injury in rats	BioFactors
眼睛
1	角膜	siRNA	CD25 siRNA induces Treg/Th1 cytokine expression in rat corneal transplantation models	Experimental Eye Research
2	视网膜	miRNA mimic	Inhibitory role of miR-203 in the angiogenesis of mice with pathological retinal neovascularization disease through downregulation of SNAI2	Cellular Signalling
鼻
1	鼻炎	miRNA mimic	MicroRNA-182-5p relieves murine allergic rhinitis via TLR4/NF-κB pathway	Open Medicine
口腔
1	口腔鳞状细胞癌	siRNA	OSCC cell‑secreted exosomal CMTM6 induced M2‑like macrophages polarization via ERK1/2 signaling pathway	Cancer Immunology, Immunotherapy
心脏
1	心力衰竭	miRNA	Upregulation of miR-93 and inhibition of LIMK1 improve ventricular remodeling and alleviate cardiac dysfunction in rats with chronic heart failure by inhibiting RhoA/ROCK signaling pathway activation	AGING
2	心肌缺血	miRNA	MiR-23a Is Involved in Myocardial Ischemia/ Reperfusion Injury by Directly Targeting CX43 and Regulating Mitophagy	Inflammation
3	心肌损伤	siRNA	Role of high‐mobility group B1 in myocardial injury induced by coronary microembolization in rats	J Cell Biochem
4	病毒性心肌炎	shRNA	Short hairpin RNA targeting 2B gene of coxsackievirus B3 exhibits potential antiviral effects both in vitro and in vivo	BMC Infectious Diseases
支气管
1	支气管（哮喘）	miRNA	Divergent Roles of miR-3162-3p in Pulmonary Inflammation in Normal and Asthmatic Mice as well as Antagonism of miR-3162-3p in Asthma Treatment	Int Arch Allergy Immunol
2	支气管（哮喘）	miRNA	Interference of miR-943-3p with secreted frizzled-related proteins4 (SFRP4) in an asthma mouse model	Cell and Tissue Research
肺部
1	肺纤维化	pAAV-FTS1-GFP-miRNA	Transforming growth factor (TGF)-β1-induced miR-133a inhibits myofibroblast differentiation and pulmonary fibrosis	Cell Death and Disease
2	肺泡出血	miRNA antagomir	In Vivo Therapeutic Success of MicroRNA-155 Antagomir in a Mouse Model of Lupus Alveolar Hemorrhage	ARTHRITIS & RHEUMATOLOGY
3	肺癌	miRNA	ENTPD5 Induces Apoptosis in Lung Cancer Cells via Regulating Caspase 3 Expression	PLOS ONE
4	肺损伤	siRNA	LPS-induced vein endothelial cell injury and acute lung injury have Btk and Orai 1 to regulate SOC-mediated calcium influx	International Immunopharmacology
5	急性肺损伤	siRNA	CRBN knockdown mitigates lipopolysaccharide-induced acute lung injury by suppression of oxidative stress and endoplasmic reticulum (ER) stress associated NF-κB signaling	Biomedicine & Pharmacotherapy
6	肺动脉高压	shRNA	LncRNA Tug1 involves in the pulmonary vascular remodeling in mice with hypoxic pulmonary hypertension via the microRNA-374c-mediated Foxc1	Life Sciences
胃
1	胃癌	siRNA	CCDC65 as a new potential tumor suppressor induced by metformin inhibits activation of AKT1 via ubiquitination of ENO1 in gastric cancer	Theranostics
2	胃酸分泌	siRNA	Source of dopamine in gastric juice and luminal dopamine induced duodenal bicarbonate secretion via apical dopamine D2 receptors	The British Pharmacological Society
胰腺
1	胰腺（糖尿病）	circRNA	Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1diabetes mellitus	Theranostics
肝脏
1	肝脏炎症	miRNA agomir	Fine-tuning the expression of microRNA-155 controls acetaminophen-induced liver inflammation	International Immunopharmacology
2	肝损伤	siRNA	Astaxanthin attenuates hepatic damage and mitochondrial dysfunction in non-alcoholic fatty liver disease by upregulating the FGF21/PGC-1α pathway	The British Pharmacological Society
3	乙型肝炎	siRNA	Hepatitis B Virus Can Be Inhibited by DNA Methyltransferase 3a via Specific Zinc-Finger-Induced Methylation of the X Promoter	BIOCHEMISTRY
4	肝 (糖异生)	siRNA	Inactivation of NF-κB2 (p52) restrains hepatic glucagon response via preserving PDE4B induction	Nature Communications
5	肝癌	siRNA	MicroRNA-125b Attenuates Epithelial-Mesenchymal Transitions and Targets Stem-Like Liver Cancer Cells Through Small Mothers Against Decapentaplegic 2 and 4	HEPATOLOGY
6	急性肝衰竭	RNA mimics	Distinct effects of different doses of kaempferol on D‑GalN/LPS‑induced ALF depend on the autophagy pathway	Molecular Medicine REPORTS
7	非酒精性脂肪肝	siRNA	Loss of RIP3 initiates annihilation of high-fat diet initialized nonalcoholic hepatosteatosis: A mechanism involving Toll-like receptor 4 and oxidative stress	Free Radical Biology and Medicine
肠
1	结直肠	miRNA mimic	Fusobacterium nucleatum promotes chemoresistance to 5-fluorouracil by upregulation of BIRC3 expression in colorectal cancer	Cancer Letters
2	结直肠	siRNA	Fusobacterium nucleatum promotes chemoresistance to 5-fluorouracil by upregulation of BIRC3 expression in colorectal cancer	Journal of Experimental & Clinical Cancer Research
3	结肠	siRNA	Effect of vasopressin on Na⁺–K⁺–2Cl^–cotransporter(NKCC)and the signaling mechanisms on the murine late distal colon	European Journal of Pharmacology
4	肠道	siRNA	Advanced oxidation protein products induce G1 phase arrest in intestinal epithelial cells via a RAGE/CD36-JNK-p27kip1 mediated pathway	Redox Biology
骨
1	软骨肉瘤	siRNA	BMPR2-pSMAD1/5 signaling pathway regulates RUNX2 expression and impacts the progression of dedifferentiated chondrosarcoma	Am J Cancer Res
2	骨形成	miRNA	miR-129-5p Inhibits Bone Formation Through TCF4	Frontiers in Cell and Developmental Biology
3	骨质疏松	shRNA	Extracellular regulated kinase 5 mediates osteoporosis through modulating viability and apoptosis of osteoblasts in ovariectomized rats	Bioscience Reports
4	骨坏死	siRNA	TET3 mediates alterations in the epigenetic marker 5hmC and Akt pathwayin steroid-associated osteonecrosis†	Journal of Bone and Mineral Research
5	骨关节炎	miRNA mimic	Upregulation of miR-98 Inhibits Apoptosis in Cartilage Cells in Osteoarthritis	GENETIC TESTING AND MOLECULAR BIOMARKERS
肌肉
1	肌肉增殖	circRNA	CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway	Cell Death & Disease
2	骨骼肌萎缩	miRNA	MiR-206 Attenuates Denervation-Induced Skeletal Muscle Atrophy in Rats Through Regulation of Satellite Cell Differentiation via TGF-b1, Smad3, and HDAC4 Signaling	Med Sci Monit
3	肌肉生成	siRNA	HMGB2 Regulates Satellite Cell-Mediated Skeletal Muscle Regeneration via IGF2BP2	Journal of Cell Science
肾脏
1	肾纤维化	miRNA	Regulation of connective tissue growth factor expression by miR-133b for the treatment of renal interstitial fibrosis in aged mice with unilateral ureteral obstruction	Stem Cell Research & Therapy
2	肾细胞癌	siRNA	Long non-coding RNA PCAT1 drives clear cell renal cell carcinoma by upregulating YAP via sponging miR-656 and miR-539	Cell Cycle
3	肾炎	miRNA agomir	MiR-130b ameliorates murine lupus nephritis through targeting type I interferon pathway on resident renal cells	Arthritis & Rheumatology
4	肾损伤	miRNA	miR-211 alleviates ischaemia/reperfusion-induced kidney injury by targeting TGFβR2/TGF-β/SMAD3 pathway	Bioengineered
膀胱
1	膀胱癌	siRNA	Autophagy promotes oncolysis of an adenovirus expressing apoptin in human bladder cancer models	Investigational New Drugs
乳腺
1	乳腺癌	miRNA inhibitor	ZLM‑7 inhibits the occurrence and angiogenesis of breast cancer through miR‑212‑3p/Sp1/VEGFA signal	Molecular Medicine
2	乳腺癌	shRNA	Short hairpin RNA targeting 2B gene of coxsackievirus B3 exhibits potential antiviral effects both in vitro and in vivo	BMC Infectious Diseases
生殖系统
1	卵巢癌	shRNA	LncRNA MAFG-AS1 promotes the malignant phenotype of ovarian cancer by upregulating NFKB1-dependent IGF1	Cancer Gene Therapy
2	睾丸生殖细胞肿瘤	miRNA antagomir	A miR-125b/CSF1-CX3CL1/tumorassociated macrophage recruitment axis controls testicular germ cell tumor growth	Cell Death and Disease
腹膜
1	腹膜纤维化	miRNA inhibitor	Long noncoding RNA AK089579 inhibits epithelial-to-mesenchymal transition of peritoneal mesothelial cells by competitively binding to microRNA-296-3p via DOK2 in peritoneal fibrosis	The FASEB Journal
免疫系统
1	红斑狼疮	miRNA	LncRNA MIAT enhances systemic lupus erythematosus by upregulating CFHR5 expression via miR-222 degradation	Experimental immunology
2	肥大细胞荨麻疹	siRNA	SELE Downregulation Suppresses Mast Cell Accumulation to Protect against Inflammatory Response in Chronic Idiopathic Urticaria	Int Arch Allergy Immunol
3	自身免疫性脑脊髓炎	miRNA	miR‑20a suppresses Treg differentiation by targeting Map3k9 in experimental autoimmune encephalomyelitis	Journal of Translational Medicine
皮肤
1	疤痕	miRNA	miR-29b promotes skin wound healing and reduces excessive scar formation by inhibition of TGF-β1/Smad/CTGF signaling pathway	Canadian Journal of Physiology and Pharmacology
其他动物
1	七鳃鳗	miRNA mimics, inhibitor	MicroRNA expression profile in Lampetra morii upon Vibrio anguillarum infection and miR-4561 characterization targeting lip	COMMUNICATIONS BIOLOGY
2	海参	dsRNA	Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber Apostichopus japonicus	Frontiers in Genetics
3	鸡胚	siRNA	lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway	Molecular Therapy: Nucleic Acids
4	飞蝗	miRNA	The microRNAs let-7 and miR-278 regulate insect metamorphosis and oogenesis by targeting the juvenile hormone early-response gene Krüppel-homolog 1	The Company of Biologists Ltd
5	幼蟹	siRNA	Molecular characteristics and abundance of insulin-like androgenic gland hormone and effects of RNA interference in Eriocheir sinensis	Animal Reproduction Science
6	半滑舌鳎鱼	siRNA	Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossussemilaevis) spleen identifies cellular processes essential to viral infection	Developmental and Comparative Immunology
7	猪	siRNA	Coronary Microembolization Induces Cardiomyocyte Apoptosis in Swine by Activating the LOX-1-Dependent Mitochondrial Pathway and Caspase-8-Dependent Pathway	Journal of Cardiovascular Pharmacology and Therapeutics

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运输与储存

常温运输，于4℃长期存储，有效期12个月。

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RNA体内转染Entranster^TM-in vivo