产品介绍
EntransterTM-H4000是英格恩生物公司研发合成的纳米聚合物转染试剂,该试剂采用纳米技术合成,是最新一代非病毒转染试剂。由于纳米技术的应用,EntransterTM-H4000在细胞转染过程中,表现了卓越的低毒、高效的性能。
本品推荐用于常规DNA以及长片段(>1kb)的mRNA等RNA的转染。
订单满1000元,免运费
EntransterTM-H4000是英格恩生物公司研发合成的纳米聚合物转染试剂,该试剂采用纳米技术合成,是最新一代非病毒转染试剂。由于纳米技术的应用,EntransterTM-H4000在细胞转染过程中,表现了卓越的低毒、高效的性能。
本品推荐用于常规DNA以及长片段(>1kb)的mRNA等RNA的转染。
常规DNA以及长片段(>1kb)的mRNA等RNA的转染。
人源,遗传变异细胞和正常组织来源细胞 | 人源,肿瘤细胞 | 其他动物来源细胞 |
AsPC-1,BNL CL2,HEK293,CFPEo,HUVEC,HLMEC,H-MVEC,MRC-5,HCS-2/8,HLF,D407,H9C2,SMC | A549,HuH-7,HepG2,HME4,A375,Hep-2,SW480,SKOV-3,IGROV1,K562,Jurkat,MCF7,MDA-MB-231,ZR-75-1,TCA8113,Eca-109,SGC-7901,HeLa,SiHa,Ca Ski,CaCo2,SAOS-2,PC-3,SHEP | B16BL6,B16F10,C2C12,C6,CHO,COS-7,CV-1,BHK-21,MDCK,PC12,BAEC,MLE-12,MLE-15,Neuro2A,PGHAM-1,RAW 264.7,EL-4,L929,LLC,3LL,NIH 3T3 |
各细胞系详细转染效率情况
细胞名称 | 来源 | 转染效率(%) |
AsPC-1 | 人源,胰脏细胞 | 80% |
BNL CL2 | 人源,胚胎肾细胞 | 70% |
HEK 293 | 人源,胚胎肾细胞 | 90% |
CFPEo | 人源,气管上皮细胞 | 50% |
HUVEC | 人源,脐带静脉内皮状细胞 | 55% |
HLMEC | 人源,肺血管内皮细胞 | 60% |
H-MVEC | 人源,微血管内皮细胞 | 50% |
MRC-5 | 人源,胚胎肺细胞 | 75% |
HCS-2/8 | 人源,软骨样细胞 | 40% |
HLF | 人源,肺成纤维细胞 | 65% |
D 407 | 人源,视网膜色素上皮细胞 | 60% |
A549 | 人源,肺癌细胞 | 90% |
HuH-7 | 人源,肝癌细胞 | 63% |
HepG2 | 人源,肝癌细胞 | 30% |
HME4 | 人源,黑色素瘤细胞 | 90% |
A375 | 人源,黑色素瘤细胞 | 70% |
Hep-2 | 人源,喉癌细胞 | 70% |
SAOS-2 | 人源,骨肉瘤细胞 | 50% |
SKOV-3 | 人源,卵巢腺癌细胞 | 60% |
IGROV1 | 人源,卵巢癌细胞 | 20% |
K562 | 人源,慢性白血病细胞 | 30% |
Jurkat | 人源,T淋巴细胞白血病细胞 | 45% |
MCF7 | 人源,乳腺癌细胞 | 55% |
MDA-MB-231 | 人源,乳腺癌细胞 | 70% |
ZR-75-1 | 人源,乳腺癌细胞 | 70% |
TCA8113 | 人源,舌鳞癌细胞 | 90% |
Eca-109 | 人源,胃腺癌细胞 | 57% |
SGC-7901 | 人源,胃腺癌细胞 | 48% |
BGC-823 | 人源,胃腺癌细胞 | 60% |
Panc-1 | 人源,胰腺癌细胞 | 40% |
HeLa | 人源,宫颈癌细胞 | 80% |
SiHa | 人源,宫颈癌细胞 | 70% |
Ca Ski | 人源,宫颈表皮样癌细胞 | 80% |
CaCo2 | 人源,结肠癌上皮细胞 | 30% |
SW480 | 人源,结肠腺癌细胞 | 80% |
PC-3 | 人源,前列腺癌细胞 | 30% |
SHEP | 人源,神经母细胞瘤细胞 | 70% |
B16BL6 | 小鼠,黑色素瘤细胞 | 90% |
B16F10 | 小鼠,黑色素瘤细胞 | 80% |
C2C12 | 小鼠,胚胎肌原细胞 | 30% |
C6 | 大鼠,脑胶质瘤细胞 | 60% |
CHO | 中国仓鼠,卵巢细胞 | 90% |
COS-7 | 猴SV40转化肾细胞 | 80% |
CV-1 | 非洲绿猴肾细胞 | 70% |
BHK-21 | 仓鼠,肾细胞 | 80% |
MDCK | 犬,肾上皮细胞 | 30% |
PC12 | 大鼠,肾上腺嗜铬细胞瘤细胞 | 33% |
BAEC | 牛,动脉内皮细胞 | 50% |
MLE-12 | 小鼠,肺上皮细胞 | 70% |
MLE-15 | 小鼠,肺上皮细胞 | 70% |
Neuro2A | 小鼠,成神经瘤细胞 | 70% |
PGHAM-1 | 仓鼠,胰腺癌细胞 | 90% |
RAW 264.7 | 小鼠,巨噬细胞 | 15% |
EL-4 | 小鼠,胸腺瘤细胞 | 75% |
L929 | 小鼠,成纤维肉瘤细胞 | 60% |
LLC | 小鼠,路易斯肺癌细胞 | 75% |
3LL | 小鼠,路易斯肺癌细胞 | 65% |
NIH 3T3 | 小鼠,胚胎纤维原细胞 | 40% |
P19 | 小鼠,胚胎肉瘤细胞 | 60% |
用法用量和常见的脂质体试剂完全一样,可不改流程方法更换试剂 转染效率更高,每支节省1000元以上,可根据需要定做500ml以上大包装,可批量定制产品。
J Virol. 2024 Nov 27:e0151124.doi: 10.1128/jvi.01511-24. Unraveling dual fusion mechanisms in BmNPV GP64: critical roles of CARC motifs and signal peptide retention 解开BmNPV GP64中的双重融合机制:CARC基序和信号肽保留的关键作用
Biol Res. 2024 Sep 9;57(1):64.doi: 10.1186/s40659-024-00544-8. Mouse testicular macrophages can independently produce testosterone and are regulated by Cebpb 小鼠睾丸巨噬细胞可以独立产生睾酮,并受Cebpb的调节
Int J Biol Macromol. 2024 May;266(Pt 1):131197.doi: 10.1016/j.ijbiomac.2024.131197. Epub 2024 Mar 28. Bombyx mori triose-phosphate transporter protein inhibits Bombyx mori nucleopolyhedrovirus infection by reducing the cell glycolysis pathway 家蚕三糖磷酸转运蛋白通过减少细胞糖酵解途径抑制家蚕核型多角体病毒感染
Invest Ophthalmol Vis Sci. 2024 Apr 1;65(4):1.doi: 10.1167/iovs.65.4.1. Identification of Novel FZD4 Mutations in Familial Exudative Vitreoretinopathy and Investigating the Pathogenic Mechanisms of FZD4 Mutations 家族性渗出性玻璃体视网膜病中新型FZD4突变的鉴定及FZD4突变致病机制的研究
Int Immunopharmacol. 2024 Apr 20:131:111864.doi: 10.1016/j.intimp.2024.111864. Epub 2024 Mar 13. miR-186-5p improves alveolar epithelial barrier function by targeting the wnt5a/β-catenin signaling pathway in sepsis-acute lung injury miR-186-5p通过靶向wnt5a/β-catenin信号通路改善脓毒症急性肺损伤肺泡上皮屏障功能
J Exp Clin Cancer Res. 2024 Apr 23;43(1):123.doi: 10.1186/s13046-024-03048-1. Tumor-suppressive miR-4732-3p is sorted into fucosylated exosome by hnRNPK to avoid the inhibition of lung cancer progression (IF:12.658).肿瘤抑制性miR-4732-3p通过hnRNPK分类为岩藻糖基外泌体,以避免对肺癌癌症进展的抑制
Mol Genet Genomics. 2024 Mar 13;299(1):32.doi: 10.1007/s00438-024-02128-3. Characterization of a novel heterozygous frameshift variant in NDP gene that causes familial exudative vitreoretinopathy in female patients 导致女性家族性渗出性玻璃体视网膜病变的NDP基因新型杂合移码变异的特征
Invest Ophthalmol Vis Sci. 2024 Mar 5;65(3):31.doi: 10.1167/iovs.65.3.31. Investigating the Impact of Dimer Interface Mutations on Norrin’s Secretion and Norrin/β-Catenin Pathway Activation 二聚体界面突变对Norrin分泌和Norrin/β-Catenin通路激活的影响
Dev Comp Immunol. 2024 Mar:152:105114.doi: 10.1016/j.dci.2023.105114. Epub 2023 Dec 13. Ras3 in Bombyx mori with antiviral function against B. mori nucleopolyhedrovirus 家蚕Ras3对家蚕核型多角体病毒具有抗病毒作用
FASEB J. 2024 Feb 29;38(4):e23493.doi: 10.1096/fj.202302387R. Deciphering a crucial dimeric interface governing Norrin dimerization and the pathogenesis of familial exudative vitreoretinopathy 解读Norrin二聚化的关键二聚体界面和家族性渗出性玻璃体视网膜病变的发病机制
Int J Biol Macromol. 2024 Feb;258(Pt 1):128570.doi: 10.1016/j.ijbiomac.2023.128570. Epub 2023 Dec 12. Frameshift variants in the C-terminal of CTNNB1 cause familial exudative vitreoretinopathy by AXIN1-mediated ubiquitin-proteasome degradation condensation (IF:8.2. CTNNB1 C末端的移码变异通过AXIN1介导的泛素-蛋白酶体降解缩合引起家族性渗出性玻璃体视网膜病变
Cell Commun Signal. 2024 Feb 1;22(1):93.doi: 10.1186/s12964-024-01497-x. Mechanosensitive channel of large conductance enhances the mechanical stretching-induced upregulation of glycolysis and oxidative metabolism in Schwann cells (IF:7.525). 大电导的机械敏感通道增强了机械拉伸诱导的雪旺氏细胞糖酵解和氧化代谢的上调
Small Methods. 2024 Jan 2:e2301310.doi: 10.1002/smtd.202301310. Online ahead of print. An Inducible CRISPR-dCas9-Based Transcriptional Repression System for Cancer Therapy 一种用于癌症治疗的基于诱导CRISPR-dCas9的转录抑制系统(IF:25.367).
Cell Death Dis. 2023 Nov 3;14(11):716.doi: 10.1038/s41419-023-06238-5. Inhibition of LSD1 induces ferroptosis through the ATF4-xCT pathway and shows enhanced anti-tumor effects with ferroptosis inducers in NSCLC CTNNB1新截短变异体引起家族性渗出性玻璃体视网膜病变
Mol Neurobiol. 2023 Nov 22.doi: 10.1007/s12035-023-03808-8. Mol Neurobiol . 2023 Nov 22. doi: 10.1007/s12035-023-03808-8. Online ahead of print. GSK-126 Attenuates Cell Apoptosis in Ischemic Brain Injury by Modulating the EZH2-H3K27me3-Bcl2l1 Axis GSK-126通过调节EZH2-H3K27me3-Bcl2l1轴减轻缺血性脑损伤中的细胞凋亡
Int J Biol Macromol. 2023 Sep 7:126801.doi: 10.1016/j.ijbiomac.2023.126801. Online ahead of print. Neddylation-dependent Neddylation-dependent LSD1 destabilization inhibits the stemness and chemoresistance of gastric cancer LSD1失稳抑制癌症的干燥和化疗耐药性
Gene. 2023 Sep 25:881:147626.doi: 10.1016/j.gene.2023.147626. Epub 2023 Jul 8. BmINR and BmAC6 genes involve in diapause regulation via the insulin/IGF signaling pathway in the silkworm (Bombyx mori) 家蚕BmINR和BmAC6基因通过胰岛素/IGF信号通路参与滞育调控
Sci Adv. 2023 Jul 21;9(29):eadf7858.doi: 10.1126/sciadv.adf7858. Epub 2023 Jul 21. Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels 工程外泌体在体内重新编程Gli1+细胞,以防止血管移植物和自体病理血管钙化 (IF:14.98).
Mol Biol Rep. 2023 Jun;50(6):5295-5306.doi: 10.1007/s11033-023-08489-z. Epub 2023 May 6. m6A-dependent mevalonate kinase in juvenile hormone synthesis pathway regulates the diapause process of bivoltine silkworm (Bombyx mori) m6A依赖性甲羟戊酸激酶在幼激素合成途径中调节二伏蚕滞育过程
Food Sci Biotechnol. 2023 Jun 19;33(2):465-474.doi: 10.1007/s10068-023-01358-2. eCollection 2024 Jan. A novel protein extracted from Hemerocallis citrina Borani inhibits hepatocellular carcinoma cell proliferation by regulating mitochondria-dependent apoptosis and aerobic glycolysis 从萱草中提取的一种新蛋白通过调节线粒体依赖性细胞凋亡和抑制肝癌细胞增殖
Insects. 2023 Apr 5;14(4):362.doi: 10.3390/insects14040362. Determination of Key Components in the Bombyx mori p53 Apoptosis Regulation Network Using Y2H-Seq Y2H-Seq法测定家蚕p53细胞凋亡调控网络的关键成分
J Med Genet. 2023 Feb;60(2):174-182.doi: 10.1136/jmedgenet-2021-108259. Epub 2022 Mar 31. Novel truncating variants in CTNNB1 cause familial exudative vitreoretinopathy CTNNB1新截短变异体引起家族性渗出性玻璃体视网膜病变
Biochem Biophys Res Commun. 2023 Jan 8:639:36-45.doi: 10.1016/j.bbrc.2022.11.067. Epub 2022 Nov 24. Silencing long non-coding RNA SNHG3 repairs the dysfunction of pulmonary microvascular endothelial barrier by regulating miR-186-5p/Wnt axis 沉默长非编码RNA SNHG3通过调节miR-186-5p/Wnt轴修复肺微血管内皮屏障功能障碍
Arch Pharm Res. 2023 Jan;46(1):44-58.doi: 10.1007/s12272-023-01426-5. Epub 2023 Jan 6. MEKs/ERKs-mediated FBXO1/E2Fs interaction interference modulates G1/S cell cycle transition and cancer cell proliferation MEKs/ERKs介导的FBXO1/E2Fs相互作用干扰调节G1/S细胞周期转变和癌症细胞增殖
Adipocyte. 2022 Dec;11(1):477-486.doi: 10.1080/21623945.2022.2107786. (IF3.553) MAPKs/AP-1, not NF-κB, is responsible for MCP-1 production in TNF-α-activated adipocytes 激活的脂肪细胞中MCP-1的产生
Microbiol Spectr. 2022 Aug 31;10(4):e0191322.doi: 10.1128/spectrum.01913-22. Epub 2022 Aug 8. (IF9.043) The Bombyx mori Nucleopolyhedrovirus GP64 Retains the Transmembrane Helix of Signal Peptide to Contribute to Secretion across the Cytomembrane 家蚕核多角体病毒GP64保留信号肽的跨膜螺旋以促进跨细胞膜分泌
Research (Wash D C) . 2022 Jul 19;2022:9826426. doi: 10.34133/2022/9826426. eCollection 2022. Programming of Regulatory T Cells In Situ for Nerve Regeneration and Long-Term Patency of Vascular Grafts. (IF:11.036).
Int J Mol Sci. 2022 Jul 2;23(13):7385.doi: 10.3390/ijms23137385. (IF6.208) CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation CDK1通过抑制∆Np63α介导的转录调节促进头颈部鳞状细胞的上皮-间充质转移和迁移
J Med Genet. 2022 Mar 31;jmedgenet-2021-108259. doi: 10.1136/jmedgenet-2021-108259. Online ahead of print. Novel truncating variants in CTNNB1 cause familial exudative vitreoretinopathy (IF:6.318).
Food Funct. 2022 Mar 7;13(5):2913-2924. doi: 10.1039/d1fo02755g. and bone formation via activating the Wnt/β-catenin signaling pathway (IF:5.396).
Ticks Tick Borne Dis. 2022 Jan 17;13(3):101906.Differential characteristics of mammalian and tick-derived promoters to trigger protein expression in transfected tick cell lines.
Dev Comp Immunol. 2022 Jun;131:104382. doi: 10.1016/j.dci.2022.104382. Epub 2022 Mar 1. A cypovirus encoded microRNA negatively regulates the NF-κB pathway to enhance viral multiplication in Silkworm, Bombyx mori
Exp Mol Med.2022 Jan;54(1):35-46.doi: 10.1038/s12276-021-00721-9.Epub 2022 Jan 12. FBXW7-mediated ERK3 degradation regulates the proliferation of lung cancer cells.
iScience. 2022 Feb 21;25(3):103949. doi: 10.1016/j.isci.2022.103949. eCollection 2022 Mar 18. Long noncoding RNA Lnc-DIF inhibits bone formation by sequestering miR-489-3p (IF:6.007).
Eur J Ophthalmol. 2022 Jan 17;11206721221074209. doi: 10.1177/11206721221074209. Online ahead of print. Whole-Exome Sequencing Reveals Novel NDP Variants in X-Linked Familial Exudative Vitreoretinopathy
J Anim Sci Biotechnol. 2021 Dec 13;12(1):120. doi: 10.1186/s40104-021-00639-2. Transcriptome sequencing analysis for the identification of stable lncRNAs associated with bovine Staphylococcus aureus mastitis (IF:5.032).
Insect Mol Biol. 2021 Dec;30(6):552-565.doi: 10.1111/imb.12725. Epub 2021 Jul 30. Functional analysis of a putative Bombyx mori cypovirus miRNA BmCPV-miR-10 and its effect on virus replication 家蚕cypovirus miRNA BmCPV-miR-10的功能分析及其对病毒复制的影响
Biomed Pharmacother. 2021 Oct;142:112056.doi:10.1016/j.biopha.2021.112056. Epub 2021 Aug 23. Dysregulation of miR-342-3p in plasma exosomes derived from convalescent AMI patients and its consequences on cardiac repair恢复期AMI患者血浆外小体中miR-342-3p的失调及其对心脏修复的影响
Fish Shellfish Immunol. 2021 Dec;119:173-181. doi: 10.1016/j.fsi.2021.09.016. Epub 2021 Oct 2. Identification and characterization of an apoptosis-inducing factor 1 involved in apoptosis and immune defense of oyster, Crassostrea gigas
Anal Chem. 2021 Aug 31;93(34):11775-11784.doi: 10.1021/acs.analchem.1c02197. Epub 2021 Aug 19. Facile Method for Specifically Sensing Sphingomyelinase in Cells and Human Urine Based on a Ratiometric Fluorescent Nanoliposome Probe 基于定量荧光纳米脂质体探针的简便特异性检测细胞和人尿液中鞘氨醇酶的方法
Cell Biosci. 2021 Aug 4;11(1):156.doi: 10.1186/s13578-021-00669-3. Modaline sulfate promotes Oct4 expression and maintains self-renewal and pluripotency of stem cells through JAK/STAT3 and Wnt signaling pathways通过JAKSTAT3和Wnt检测干细胞
Front Physiol. 2021 Aug 4;12:663482.doi:10.3389/fphys.2021.663482. eCollection 2021. Two Putative Cypovirus-Encoded miRNAs Co-regulate the Host Gene of GTP-Binding Nuclear Protein Ran and Facilitate Virus ReplicationmiRNA共同调节宿主基因GTP结合核蛋白Ran的表达
Cell Death Differ. 2021 Jul;28(7):2160-2178.doi:10.1038/s41418-021-00744-9. Epub 2021 Mar 4. MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasmMACF1通过隔离促进成骨细胞分化
Genet Test Mol Biomarkers. 2021 Jun;25(6):399-404.doi: 10.1089/gtmb.2021.0019. Epub 2021 Jun 2.Whole-Exome Sequencing Reveals Novel TSPAN12 Variants in Autosomal Dominant Familial Exudative Vitreoretinopathy
Front Pharmacol. 2021 Jun 14;12:696729.doi:10.3389/fphar.2021.696729. eCollection 2021. Forsythiasides-Rich Extract From Forsythiae Fructus Inhibits Mast Cell Degranulation by Enhancing Mitochondrial Ca 2+ Uptake连翘抑制肥大细胞生长线粒体钙摄取
Biochem Pharmacol. 2021 Apr;186:114476.doi:10.1016/j.bcp.2021.114476. Epub 2021 Feb 17. Aloe-emodin, a naturally occurring anthraquinone, is a highly potent mast cell stabilizer through activating mitochondrial calcium uniporter芦荟大黄素
Gene. 2021 Apr 20;777:145450.doi: 10.1016/j.gene.2021.145450. Epub 2021 Jan 29. A 14-amino acids deletion in BmShadow results to non-moult on the 2nd instar in the bivoltine silkworm, Bombyx moriBmShadow中14个氨基酸的缺失导致二伏家蚕2龄时不蜕皮
FEBS Open Bio. 2021 Mar;11(3):890-897.doi: 10.1002/2211-5463.13109. Epub 2021 Feb 19. Pin1 and JNK1 cooperatively modulate TAp63γ Pin1和JNK1协同调节TAp63γ
Pharmacol Res. 2021 Jan;163:105230.doi:10.1016/j.phrs.2020.105230. Epub 2020 Oct 5. Long noncoding RNA AK039312 and AK079370 inhibits bone formation via miR-199b-5p长非编码RNA AK039312和AK079370抑制骨形成
Cell Death Differ. 2021 Jan;28(1):337-348.doi: 10.1038/s41418-020-00620-y. Epub 2020 Sep 9. RPS23RG1 modulates tau phosphorylation and axon outgrowth through regulating p35 proteasomal degradation RPS23RG1通过调节p35蛋白酶体降解调节tau磷酸化和轴突生长
Pharmacol Res. 2020 Oct 5;105230. doi: 10.1016/j.phrs.2020.105230 Long noncoding RNA AK039312 and AK079370 inhibits bone formation via miR-199b-5p
Biochem Biophys Res Commun. 2020 Dec10;533(3):613-619.doi:10.1016/j.bbrc.2020.09.063. Epub 2020 Sep 25. Prefoldin subunit MM1 promotes cell migration via facilitating filopodia formation预折叠蛋白亚单位MM1通过促进细胞迁移
Graefes Arch Clin Exp Ophthalmol. 2020 Oct;258(10):2251-2261.doi: 10.1007/s00417-020-04636-5.Epub 2020 Jun 7.Disease-causing mutations associated with bestrophinopathies promote apoptosis in retinal pigment epithelium cells.视网膜色素上皮细胞凋亡
Biochem Pharmacol. 2020 Jul;177:113984.doi:10.1016/j.bcp.2020.113984. Epub 2020 Apr 18. Ethyl-p-methoxycinnamate enhances oct4 expression and reinforces pluripotency through the NF-κB signaling pathway对甲氧基肉桂酸乙酯增强oct4表达并增强多能性
Small.2020 Jul;16(30):e1907301.doi: 10.1002/smll.201907301. Epub 2020 Jun 25.Photoactivatable RNA N 6 -Methyladenosine Editing with CRISPR-Cas13甲基腺苷编辑(IF:13.281).
Small. 2020 Jul;16(30):e1907301.doi: 10.1002/smll.201907301. Epub 2020 Jun 25. Photoactivatable RNA N6 -Methyladenosine Editing with CRISPR-Cas13 CRISPR-Cas13编辑可光激活RNA N6-甲基腺苷 (IF:13.3).
Front Genet.2020 May 26;11:511.doi: 10.3389/fgene.2020.00511. eCollection 2020.MICA ∗ 012:01 Allele Facilitates the Metastasis of KRAS-Mutant Colorectal Cancer.KRAS突变型结直肠癌的转移
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EntransterTM系列转染试剂的成分和原理,化学结构,粒径,电位等数据
siRNA转染与DNA转染有什么不一样?
为什么EntransterTM转染试剂有血清转染不仅没有毒性,反而有助于提高转染效率?
为什么EntransterTM系列转染试剂转染时可以有抗生素,而脂质体转染对抗生素敏感?
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