Measuring Laplace pressure and imaging the actin cortex during cytokinesis in cultured cells

Xiaohuan Wang^{1 2}, Long Li¹, Fan Song^{1 2}

Affiliations

¹ State Key Laboratory of Nonlinear Mechanics and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China.
² School of Engineering Science, University of Chinese Academy of Sciences, Beijing 100049, China.

PMID: 35310072
PMCID: PMC8931440
DOI: 10.1016/j.xpro.2022.101239

Abstract

Laplace pressure is an important regulator of cell dynamics and behavior during cytokinesis. Here, we provide a protocol to measure Laplace pressure in cultured cells using a micropipette and describe the steps for imaging the actin cortex during cytokinesis. The quantification steps allow tracing dynamic change in Laplace pressure and displaying dynamic response of the actin cortex during cytokinesis in HeLa cells. This protocol can be applied to any cultured cell type during various stages of cell division. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).

Keywords: Biophysics; Cell Biology; Cell culture; Cell-based Assays; Microscopy; Single Cell.

STAR Protoc. 2022 Mar 15;3(2):101239.doi: 10.1016/j.xpro.2022.101239. eCollection 2022 Jun 17.

Measuring Laplace pressure and imaging the actin cortex during cytokinesis in cultured cells

Abstract

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