本文采用的英格恩产品: RNA-Entranster-invivo

MicroRNA expression profile in Lampetra morii upon Vibrio anguillarum infection and miR-4561 characterization targeting lip

Lie Ma ^{#  1   2} , Meng Gou ^{#  1   2} , Zeyu Du ^{#  1   2} , Ting Zhu ^{#  1   2} , Jun Li  ^{1   2} , Qing Wei Li  ^{1   2} , Yue Pang  ^{3   4} Affiliations

• PMID: 34417547
• PMCID: PMC8379177
• DOI: 10.1038/s42003-021-02525-z

Free PMC article

Abstract

As a critical evolutionary pivot between invertebrates and vertebrates, lampreys provide rich genetic information. Lamprey immune protein (LIP) is a key immune regulator. MicroRNAs, well-conserved in the response to immunological stress, remain understudied in lamprey immunity. We generated a lamprey microRNA expression atlas, using deep sequencing, upon Vibrio anguillarum infection. Using comparative methods, we found that miR-4561 potentially regulates innate immunity via interaction with lip. We found a sequence in the 3′-UTR region of LIP mRNA complementary to the miR-4561 seed region; miR-4561 expression was negatively correlated with LIP. During V. anguillarum infection, miR-4561 inhibited LIP expression and bacterial clearance. Notably, LIP expression in supraneural body cells was necessary for the Gram-negative immune response. Additionally, we observed that overexpression of miR-4561 induced apoptosis in embryonic cells, suggesting a role in embryonic development. Collectively, we show lamprey microRNAs may significantly affect gene regulation and provide new insights on LIP-mediated immune regulation.