本文采用的英格恩产品: RNA-Entranster-invivo

Fusobacterium nucleatum promotes chemoresistance to 5-fluorouracil by upregulation of BIRC3 expression in colorectal cancer

Sheng Zhang  ^{1   2} , Yongzhi Yang  ^{1   2} , Wenhao Weng  ^{3   4} , Bomin Guo  ⁵ , Guoxiang Cai  ^{1   2} , Yanlei Ma  ^{6   7} , Sanjun Cai  ^{8   9}

Affiliations

• ¹ Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, 270 Dong’an Road, Shanghai, 200032, China.
• ² Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
• ³ Department of Clinical Laboratory, Yangpu Hospital, Tongji University School of Medicine, Shanghai, China.
• ⁴ Center for Translational Medicine, Yangpu Hospital, Tongji University School of Medicine, Shanghai, China.
• ⁵ Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China.
• ⁶ Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, 270 Dong’an Road, Shanghai, 200032, China. yanleima@fudan.edu.cn.
• ⁷ Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. yanleima@fudan.edu.cn.
• ⁸ Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, 270 Dong’an Road, Shanghai, 200032, China. sanjuncai@hotmail.com.
• ⁹ Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. sanjuncai@hotmail.com.

Abstract

Background: Emerging evidence suggests a potential relationship between gut microbiota and the host response to chemotherapeutic drugs including 5-fluorouracil (5-Fu). Fusobacterium nucleatum (Fn) has been linked to the initiation and progression of colorectal cancer (CRC). Unfortunately, little was known about the relationship between Fn infection and chemotherapeutic efficacy. Here, we investigate the potential relationship between Fn infection and chemotherapeutic efficacy of 5-Fu in CRC.

Methods: Differentially expressed genes of CRC cell lines induced by Fn infection were analyzed based on a whole genome microarray analysis Then, we explored the relationship between upregulation of BIRC3 induced by Fn infection and chemoresistance to 5-Fu in vitro and in vivo. Furthermore, we dissected the mechanisms involved in Fn-induced BIRC3 expression. Finally, we investigated the clinical relevance of Fn infection, BIRC3 protein expression and chemoresistance to 5-Fu treatment in CRC patients.

Results: BIRC3 was the most upregulated gene induced by Fn infection via the TLR4/NF-κB pathway in CRC cells; Fn infection reduced the chemosensitivity of CRC cells to 5-Fu through upregulation of BIRC3 in vitro and in vivo. High Fn abundance correlated with chemoresistance in advanced CRC patients who received standard 5-Fu-based adjuvant chemotherapy after radical surgery.

Conclusions: Our evidence suggests that Fn and BIRC3 may serve as promising therapeutic targets for reducing chemoresistance to 5-Fu treatment in advanced CRC.

Keywords: 5-fluorouracil; BIRC3; Chemoresistance; Colorectal cancer; Fusobacterium nucleatum.